RoSane

Synaptotagmin is a synaptic vesicle protein that regulates neurotransmission. The poorly studied intravesicular domain of this protein is transitorily exposed to the extracellular milieu upon exocytosis. We recently identified a direct interaction of this domain with glycolipids of the neuronal plasma membrane extracellular leaflet. Intriguingly this interaction is also important for bacterial neurotoxins recognition of neuronal membranes. Using biochemical and structural methods as well as imaging and electrophysiological recordings, this project aims at understanding the implication of this interaction in the exo-endocytotic cycle of synaptic vesicles.