NeuroInflamDyn

This application which gathers neurobiologists and immunologists has for objective to analyze the interdependence of inflammation, neurodegeneration and disease progression in multiple sclerosis. As a model, experimental autoimmune encephalomyelitis, which recapitulates the pathological hallmarks of MS, is induced in Thy1-CFP//LysM-EGFP//CD11c-EYFP triple mutant reporter mice. We will combine our recent methodological developments in (1) advanced spinal cord in vivo 2 photon spectral imaging, (2) high content flow cytometry analysis and (3) mouse genetics to (1) characterize imaging markers of disease progression, (2) obtain a global picture of the immune cells in spinal tissue over time as well as (3) a functional characterization of the dendritic cells/monocytes subtypes involved, (4) explore the effect of VEGF on the immune signature to provide the proof that the methodology is suited to perform refined drug testing.