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Completed
14-3-3 Membrane Biology: Central Element in Signal Transduction Networks
FunderNetherlands Organisation for Scientific Research (NWO)
Duration01 Sep 2008 - 09 Sep 2013
Project code817.02.026
Open Access mandatePublications: NoResearch data: No
Identifiers
Project timeline100%
Description
This study aims to characterize the importance of interaction between membrane (associated) proteins and the family of 14-3-3 proteins in Arabidopsis thaliana L. Membrane proteins are instrumental in ion homeostasis and form an integral part of hormonal and stress induced signal transduction networks. Despite their importance, our understanding of how these proteins are regulated at the post-translational level is still far from complete. In recent years evidence has accumulated that 14-3-3 proteins control a range of integral membrane proteins and membrane associated proteins: ion channels, ion pumps, receptor kinases and signal amplifiers like G-proteins, PLD and NADPH-oxidase. Here, we propose to make a comprehensive study of the role of 14-3-3 proteins in plant membrane biology. 14-3-3 proteins act through phosphorylation-dependent interaction with target proteins, thereby altering their activity or their subcellular localization. With the identification of hundreds of cellular targets, it becomes clear now that 14-3-3 target proteins often cluster in functional networks like apoptosis, cell cycle, carbon metabolism etc. The challenge, central to this project, is to find out whether 14-3-3 proteins play such a coordinating role in the abscisic acid (ABA) signaling network, with a focus on guard cells, as recently described (Li et al. 2006; [26]). The project follows four consecutive steps: i) targeted screening of (families of) membrane (associated) proteins for interaction with the 14-3-3 protein family using the yeast two-hybrid system. ii) confirming the interaction using bimolecular fluorescence complementation (BIFC), iii) functional analysis of interaction for a selected number of targets, iv) assessing the in vivo function of selected targets through the introduction of 14-3-3¯ (minus) targets in their respective mutant plants. The outcome will be the start of a network map of 14-3-3 functions in membrane biology, that will guide further research in understanding 14-3-3 membrane biology in guard cell signalling and (a)biotic stress adaptation.
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This study aims to characterize the importance of interaction between membrane (associated) proteins and the family of 14-3-3 proteins in Arabidopsis thaliana L. Membrane proteins are instrumental in ion homeostasis and form an integral part of hormonal and stress induced signal transduction networks. Despite their importance, our understanding of how these proteins are regulated at the post-translational level is still far from complete. In recent years evidence has accumulated that 14-3-3 proteins control a range of integral membrane proteins and membrane associated proteins: ion channels, ion pumps, receptor kinases and signal amplifiers like G-proteins, PLD and NADPH-oxidase. Here, we propose to make a comprehensive study of the role of 14-3-3 proteins in plant membrane biology. 14-3-3 proteins act through phosphorylation-dependent interaction with target proteins, thereby altering their activity or their subcellular localization. With the identification of hundreds of cellular targets, it becomes clear now that 14-3-3 target proteins often cluster in functional networks like apoptosis, cell cycle, carbon metabolism etc. The challenge, central to this project, is to find out whether 14-3-3 proteins play such a coordinating role in the abscisic acid (ABA) signaling network, with a focus on guard cells, as recently described (Li et al. 2006; [26]). The project follows four consecutive steps: i) targeted screening of (families of) membrane (associated) proteins for interaction with the 14-3-3 protein family using the yeast two-hybrid system. ii) confirming the interaction using bimolecular fluorescence complementation (BIFC), iii) functional analysis of interaction for a selected number of targets, iv) assessing the in vivo function of selected targets through the introduction of 14-3-3¯ (minus) targets in their respective mutant plants. The outcome will be the start of a network map of 14-3-3 functions in membrane biology, that will guide further research in understanding 14-3-3 membrane biology in guard cell signalling and (a)biotic stress adaptation.
