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Completed
Songbird genomics: High throughput genetics and QTL mapping in wild passerines
FunderNetherlands Organisation for Scientific Research (NWO)
Duration07 Apr 2008 - 26 Jan 2011
Project codeSH-088-08-V
Identifiers
Project timeline100%
Description
Met behulp van de nieuwste DNA sequence-methoden generen we miljoenen korte DNA sequenties (35 bp). Deze sequenties gebruiken we om tienduizenden sequentie-polymorphismen op te sporen in de koolmees, waarvoor het genoom van zowel de kip als de zebravink als referentiegenoom dient. De polymorphismen dienen voor het genereren van een linkage map van de koolmees. Een deel van de gevonden polymorphismen wordt gebruikt voor een screening van duizenden koolmezen van het NIOO in Heteren waarvan de persoonlijkheid en het broedgedrag bekend is. Dit zal resulteren in het mappen van QTLs, en in het identificeren van kandidaatgenen voor deze QTLs.
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Met behulp van de nieuwste DNA sequence-methoden generen we miljoenen korte DNA sequenties (35 bp). Deze sequenties gebruiken we om tienduizenden sequentie-polymorphismen op te sporen in de koolmees, waarvoor het genoom van zowel de kip als de zebravink als referentiegenoom dient. De polymorphismen dienen voor het genereren van een linkage map van de koolmees. Een deel van de gevonden polymorphismen wordt gebruikt voor een screening van duizenden koolmezen van het NIOO in Heteren waarvan de persoonlijkheid en het broedgedrag bekend is. Dit zal resulteren in het mappen van QTLs, en in het identificeren van kandidaatgenen voor deze QTLs.
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