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Completed
Modification of the expression of transcriptional regulators of Aspergillus to improve industrial fermentations and use of agricultural by-products
FunderNetherlands Organisation for Scientific Research (NWO)
Duration15 Aug 2007 - 15 Jul 2013
Project code07938
Identifiers
Project timeline100%
Description
Industry uses fungi from the genus Aspergillus for the production of metabolites and proteins. Production is often performed in large-scale fermentations using crude carbon sources, which consist of a variety of polymers such as starch, cellulose, xylan, xyloglucan, galacto(gluco)mannan, and pectin. These polymers are degraded by a large spectrum of secreted enzymes and the resulting degradation products are taken up by the fungus to serve as nutrients. In fact, these secreted enzymes are abundantly used in industry. The production of secreted proteins is known to be tightly regulated but little is known about the regulators involved. For instance, at least 10 regulators are expected to be involved in the degradation of sugar beet pulp but only 2 regulators have been reported in literature. The recent availability of the genome sequence (Pel et al, 2007) and DNA arrays of Aspergillus niger (facilitated by DSM) allow new approaches for the identification of transcriptional regulators of A. niger that are involved in pectin and galacto(gluco)mannan utilisation. These polymers are abundantly present in crude carbon sources. In this project we aim at identifying regulators and assess their role in extracting energy from crude carbon sources and the production of industrial enzymes and metabolites. In addition, the research should result in the production of high value products from agricultural by-products. Finally, we will evaluate to which extent these regulatory systems are conserved in fungi and transfer the knowledge obtained with A. niger to other industrial fungi and to phytopathogenic fungi.
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Industry uses fungi from the genus Aspergillus for the production of metabolites and proteins. Production is often performed in large-scale fermentations using crude carbon sources, which consist of a variety of polymers such as starch, cellulose, xylan, xyloglucan, galacto(gluco)mannan, and pectin. These polymers are degraded by a large spectrum of secreted enzymes and the resulting degradation products are taken up by the fungus to serve as nutrients. In fact, these secreted enzymes are abundantly used in industry. The production of secreted proteins is known to be tightly regulated but little is known about the regulators involved. For instance, at least 10 regulators are expected to be involved in the degradation of sugar beet pulp but only 2 regulators have been reported in literature. The recent availability of the genome sequence (Pel et al, 2007) and DNA arrays of Aspergillus niger (facilitated by DSM) allow new approaches for the identification of transcriptional regulators of A. niger that are involved in pectin and galacto(gluco)mannan utilisation. These polymers are abundantly present in crude carbon sources. In this project we aim at identifying regulators and assess their role in extracting energy from crude carbon sources and the production of industrial enzymes and metabolites. In addition, the research should result in the production of high value products from agricultural by-products. Finally, we will evaluate to which extent these regulatory systems are conserved in fungi and transfer the knowledge obtained with A. niger to other industrial fungi and to phytopathogenic fungi.
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Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. 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Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. 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Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. Provider Academic Bibliometrics & Assessment Computational Utility Service Expiration Session Name greekSession Description Stores the user session ID for Academic Bibliometrics & Assessment Computational Utility Service. 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