English
This Έργο is not specific to Greece. For more details, please visit OpenAIRE Explore.
Link with research outputs.
Please log in first and try again.
Completed
Ex-vivo dialysis of human kidneys for regenerative medicine
FunderNetherlands Organisation for Scientific Research (NWO)
Duration01 Sep 2019 - 21 Oct 2020
Project codeNWA.1228.191.422
Open Access mandatePublications: NoResearch data: No
Identifiers
Project timeline100%
Description
Regenerative medicine (RM), highlighted as a main route of interest by the national science agenda, could possibly benefit millions of patients with chronic kidney diseases. However, there is currently a large gap between RM developments in laboratories and the functional validation of these in human organs. Our solution to this problem is the development of an ex-vivo organ culture system, which could culture and maintain viable human kidneys outside the human body. Such an organ culture as a platform technology would allow the validation of a variety of RM therapies in a safe and modifiable environment which can be directly translated to human use. This would accelerate the road towards clinical application of RM therapies. To reach our goal we will exploit clinical dialysis technologies (continuous veno-venous hemofiltration (CCVH)) in combination with readily available customized organ chambers. Using a CCVH system will maintain ion, electrolyte and gas homeostasis within the kidney inside a culture chamber. This should allow perfusion for a prolonged period of time (up to 7-14 days) and maintain them viable and functional to ultimately investigate the regenerative medicine potential of stem cell derived nephron structures.
Partners (2)
Start a new DMP in Loading
Regenerative medicine (RM), highlighted as a main route of interest by the national science agenda, could possibly benefit millions of patients with chronic kidney diseases. However, there is currently a large gap between RM developments in laboratories and the functional validation of these in human organs. Our solution to this problem is the development of an ex-vivo organ culture system, which could culture and maintain viable human kidneys outside the human body. Such an organ culture as a platform technology would allow the validation of a variety of RM therapies in a safe and modifiable environment which can be directly translated to human use. This would accelerate the road towards clinical application of RM therapies. To reach our goal we will exploit clinical dialysis technologies (continuous veno-venous hemofiltration (CCVH)) in combination with readily available customized organ chambers. Using a CCVH system will maintain ion, electrolyte and gas homeostasis within the kidney inside a culture chamber. This should allow perfusion for a prolonged period of time (up to 7-14 days) and maintain them viable and functional to ultimately investigate the regenerative medicine potential of stem cell derived nephron structures.
